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Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?


A) Ouchterlony double diffusion
B) Western Blot
C) immunelectrophoresis
D) radioimmunoassay RIA)
E) the Quellung test

F) A) and C)
G) B) and E)

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The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.


A) lower; lower
B) higher; lower
C) lower; higher
D) higher; higher

E) None of the above
F) A) and C)

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Which test is used to measure the IgE present in allergic patients?


A) fluorescent antibody tests
B) Rapid Pla Reagin RPR) test
C) Weil-Felix reaction
D) RIST
E) ELISA

F) B) and C)
G) A) and C)

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Describe the indirect ELISA test that is used as a screening test for HIV AIDS) and include the following: a) sequence of procedures, b) reagents used, and c) detection and interpretation of a positive test.

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The Widal test is used for diagnosing salmonellosis.

A) True
B) False

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Serological testing always involves reactions between specific antibody and antigen.

A) True
B) False

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Culture of patient specimens to detect pathogens is usually done on blood agar plates only.

A) True
B) False

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In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

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Describe three possible sampling sites and methods of collection for clinical laboratories.

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1. Blood Collection from a Vein: One com...

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Pulsed-field gel electrophoresis PFGE) is often used to determine whether bacterial isolates from different cases of food-borne illness are the same and can be traced to the same source. This is an example of


A) an immunologic technique.
B) a genetic method.
C) a phenotypic method.
D) a microscopic technique.

E) A) and B)
F) All of the above

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Serological tests should have low sensitivity and specificity.

A) True
B) False

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ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

A) True
B) False

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In indirect fluorescent testing, fluorescent _____ are used to react with the Fc region of another, specific, serum antibody.

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Tests that take place in the lab, such as in a test tube or Petri-dish, outside of a living host, are referred to as _______________ whereas tests that take place in a living host, such as in a patient, are described as ______________.


A) In loco; In vitro
B) In vitro; In loco
C) In vitro; In vivo
D) In loco; In vivo

E) C) and D)
F) B) and D)

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Which technique used to diagnose a disease is also called the double diffusion method?


A) Ouchterlony test
B) VDRL test
C) immunoelectrophoresis
D) Western Blot
E) ELISA

F) D) and E)
G) None of the above

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Test systems involving which of the following has greatly improved the specificity of immunological tests?


A) Monoclonal antibodies
B) Electronics
C) Enzymes
D) Radioactivity
E) All of these choices are correct.

F) None of the above
G) C) and E)

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