Question 1

Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled, and cooled?

Accepted Answer

A)   A pure culture
B)   A mixed culture
C)   A solid medium
D)   A liquid medium
E)   A contaminated mediumF) A) and D)
G) D) and E)

Question 2

The purpose of staining cells on a microscope slide is to ________.

Accepted Answer

A)   kill them
B)   secure them to the slide
C)   enlarge the cells
D)   add contrast in order to see them better
E)   see motilityF) C) and E)
G) B) and E)

Question 3

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would likely be present in selective media for analyzing this fecal specimen?

Accepted Answer

A)   NaCl
B)   Sheep red blood cells
C)   Bile salts
D)   Thioglycollic acid
E)   PeptoneF) A) and B)
G) C) and D)

Question 4

The patient's culture results positive for Staphylococcus. The RN understands that the culture most likely required growth on a complex medium, consisting of

Accepted Answer

A)   an exact chemical formula.
B)   chemical growth inhibitors.
C)   at least one ingredient that is not chemically defined.
D)   None of the choices are correct.E) B) and D)
F) None of the above

Question 5

Which of the following is not a benefit of agar as a solid medium?

Accepted Answer

A)   Has flexibility
B)   Holds moisture
C)   Can be inoculated and poured at a temperature that is not harmful
D)   Is solid at room temperature
E)   Is digested by most microbesF) C) and E)
G) A) and E)

Question 6

Which type of microscope shows cells against a bright background but also differentiates intracellular structures of unstained cells based on their varying densities?

Accepted Answer

A)   Bright field
B)   Dark field
C)   Phase contrast
D)   Differential interference
E)   ElectronF) A) and B)
G) A) and D)

Question 7

Some microbes are not capable of growing on artificial media.

Accepted Answer

A) True 
 B)False

Question 8

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

Accepted Answer

A)   streaked
B)   poured
C)   incubated
D)   contaminated
E)   All of the choices are correct.F) A) and E)
G) A) and B)

Question 9

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Accepted Answer

A)   Streak plate
B)   Spread plate
C)   Pour plate
D)   All of the choices are correct.
E)   None of the choices are correct.F) A) and D)
G) All of the above

Question 10

The ________ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

A)   stage
B)   condenser
C)   objective
D)   ocular
E)   nosepieceF) B) and E)
G) B) and D)

Question 11

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Accepted Answer

A) True 
 B)False

Question 12

One colony typically develops from the growth of several parent bacterial cells.

Accepted Answer

A) True 
 B)False

Question 13

All of the following pertain to the fluorescence microscope except ________.

Accepted Answer

A)   it uses electrons to produce a specimen image
B)   it is a type of compound microscope
C)   it requires the use of dyes like acridine and fluorescein
D)   it is commonly used to diagnose certain infections
E)   it requires an ultraviolet radiation sourceF) B) and E)
G) None of the above

Question 14

A sputum sample is ordered for microbial analysis in order to rule out the diagnosis of tuberculosis. Suspecting Mycobacterium tuberculosis may be the pathogen, the RN knows the laboratory technicians will perform which stain on the sample?

Accepted Answer

A)   Endospore stain
B)   Negative stain
C)   Flagellar stain
D)   Acid-fast stainE) All of the above
F) None of the above

Question 15

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)   Differential
B)   Selective
C)   Enumeration
D)   Enriched
E)   ReducingF) A) and D)
G) A) and C)

Question 16

Which of the following is not an inoculating tool?

Accepted Answer

A)   Petri dish
B)   Loop
C)   Needle
D)   Pipette
E)   SwabF) A) and B)
G) A) and C)

Question 17

Comparing defined vs. complex media is analogous to comparing ________.

Accepted Answer

A)   baby formula to breast milk
B)   Coca-cola® to Pepsi®
C)   the generic version of the drug Lipitor® to the brand name produced by Pfizer Pharmaceuticals
D)   a strawberry-banana smoothie to a mixed berry smoothieE) A) and C)
F) B) and D)

Question 18

The sterile vaginal fluid specimen is sent for culture. The RN educates the patient about the five basic techniques utilized by laboratory technicians to manipulate, grow, examine, and characterize any microorganisms present in the specimen. Which of the following is the correct order of steps for processing the specimen?

Accepted Answer

A)   Isolation, incubation, inspection, identification, and inoculation
B)   Inspection, identification, isolation, incubation, and inoculation
C)   Identification, isolation, incubation, inspection, and inoculation
D)   Inoculation, incubation, isolation, inspection, and identificationE) B) and D)
F) None of the above

Question 19

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A)   Differential interference contrast
B)   Scanning electron
C)   Transmission electron
D)   Phase contrast
E)   FluorescenceF) B) and C)
G) All of the above

Question 20

Normal incubation temperatures range from 30°C to 60°C.

Accepted Answer

A) True 
 B)False

Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

One colony typically develops from the growth of several parent bacterial cells.

Correct Answer
verified

The ________ of the microscope holds and allows selection of the objective lenses.

Correct Answer
verified

Some microbes are not capable of growing on artificial media.

Correct Answer
verified

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Correct Answer
verified

Comparing defined vs. complex media is analogous to comparing ________.

Correct Answer
verified

Which type of microscope shows cells against a bright background but also differentiates intracellular structures of unstained cells based on their varying densities?

Correct Answer
verified

All of the following pertain to the fluorescence microscope except ________.

Correct Answer
verified

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

Correct Answer
verified

The purpose of staining cells on a microscope slide is to ________.

Correct Answer
verified

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Correct Answer
verified

Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled, and cooled?

Correct Answer
verified

The patient's culture results positive for Staphylococcus. The RN understands that the culture most likely required growth on a complex medium, consisting of

Correct Answer
verified

Correct Answer
verified

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct Answer
verified

Which of the following is not a benefit of agar as a solid medium?

Correct Answer
verified

Normal incubation temperatures range from 30°C to 60°C.

Correct Answer
verified

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct Answer
verified

Which of the following is not an inoculating tool?

Correct Answer
verified

A sputum sample is ordered for microbial analysis in order to rule out the diagnosis of tuberculosis. Suspecting Mycobacterium tuberculosis may be the pathogen, the RN knows the laboratory technicians will perform which stain on the sample?

Correct Answer
verified

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would likely be present in selective media for analyzing this fecal specimen?

Correct Answer
verified

Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

One colony typically develops from the growth of several parent bacterial cells.

Correct AnswerverifiedShow Answer

The ________ of the microscope holds and allows selection of the objective lenses.

Correct AnswerverifiedShow Answer

Some microbes are not capable of growing on artificial media.

Correct AnswerverifiedShow Answer

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Correct AnswerverifiedShow Answer

Comparing defined vs. complex media is analogous to comparing ________.

Correct AnswerverifiedShow Answer

Which type of microscope shows cells against a bright background but also differentiates intracellular structures of unstained cells based on their varying densities?

Correct AnswerverifiedShow Answer

All of the following pertain to the fluorescence microscope except ________.

Correct AnswerverifiedShow Answer

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

Correct AnswerverifiedShow Answer

The purpose of staining cells on a microscope slide is to ________.

Correct AnswerverifiedShow Answer

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Correct AnswerverifiedShow Answer

Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled, and cooled?

Correct AnswerverifiedShow Answer

The patient's culture results positive for Staphylococcus. The RN understands that the culture most likely required growth on a complex medium, consisting of

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct AnswerverifiedShow Answer

Which of the following is not a benefit of agar as a solid medium?

Correct AnswerverifiedShow Answer

Normal incubation temperatures range from 30°C to 60°C.

Correct AnswerverifiedShow Answer

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

Which of the following is not an inoculating tool?

Correct AnswerverifiedShow Answer

A sputum sample is ordered for microbial analysis in order to rule out the diagnosis of tuberculosis. Suspecting Mycobacterium tuberculosis may be the pathogen, the RN knows the laboratory technicians will perform which stain on the sample?

Correct AnswerverifiedShow Answer

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would likely be present in selective media for analyzing this fecal specimen?

Correct AnswerverifiedShow Answer

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