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In 1884, Hans Christian Gram described a method of staining bacterial cells while not staining surrounding animal tissues. However, he thought that the staining method he developed was faulty because not all bacteria stained. In a letter to the editor of the journal in which Gram published his findings, write your response to Gram's concern.

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Dear Hans Christian Gram,

I recently re...

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Which microscope achieves the highest magnification and greatest resolution?


A) electron microscope
B) darkfield microscope
C) phase- contrast microscope
D) compound light microscope
E) fluorescence microscope

F) A) and C)
G) A) and D)

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Bacterial smears are fixed before staining to


A) make the cells visible.
B) affix the cells to the slide.
C) make their walls permeable.
D) accept stain.

E) A) and B)
F) All of the above

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You are performing a Gram stain on gram- positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?


A) colorless
B) purple
C) brown
D) red

E) B) and C)
F) A) and D)

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You find colorless areas in cells in a gram- stained smear. What should you apply next?


A) an acid- fast stain
B) a flagella stain
C) a capsule stain
D) a simple stain
E) an endospore stain

F) A) and E)
G) None of the above

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Which microscope is used to see intracellular detail in a living cell?


A) atomic force microscope
B) two- photon microscope
C) brightfield microscope
D) fluorescence microscope
E) transmission electron microscope

F) A) and E)
G) None of the above

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You are performing a Gram stain on gram- negative bacteria and you stop after the addition of the mordant. What is the appearance of the bacteria at this point?


A) red
B) purple
C) brown
D) colorless

E) A) and B)
F) A) and C)

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Which microscope uses two beams of light to produce a three- dimensional color image?


A) DIC microscope
B) fluorescence microscope
C) electron microscope
D) phase- contrast microscope
E) darkfield microscope

F) A) and E)
G) A) and B)

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The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.

A) True
B) False

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In 1877, Robert Koch thought preparing permanently stained slides would be valuable. Why was his assessment correct?

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Answered by ExamLex AI

In 1877, Robert Koch, a pioneering micro...

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Figure 3.1 Figure 3.1    -In Figure 3.1, line  c  points to the microscope's A)  illuminator. B)  ocular lens. C)  objective lens. D)  condenser. -In Figure 3.1, line "c" points to the microscope's


A) illuminator.
B) ocular lens.
C) objective lens.
D) condenser.

E) A) and D)
F) A) and C)

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Which of the following correctly traces the path of light through the compound microscope?


A) light source; condenser; objective lens; specimen; ocular lens
B) light source; condenser; specimen; objective lens; ocular lens
C) condenser; light source; specimen; objective lens; ocular lens
D) condenser; light source; specimen; ocular lens; objective lens
E) light source; specimen; condenser; objective lens; ocular lens

F) All of the above
G) A) and E)

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What structure does light pass through after leaving the condenser in a compound light microscope?


A) specimen
B) illuminator
C) objective lens
D) ocular lens

E) None of the above
F) B) and D)

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A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear


A) larger than it would if immersion oil was used.
B) to have no color.
C) the same as it would if the immersion oil was used.
D) smaller than it would if immersion oil was used.
E) somewhat fuzzy and have poor resolution.

F) A) and E)
G) B) and D)

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Which microscope is used to observe viruses and the internal structure of thinly sectioned cells?


A) fluorescence microscope
B) scanning electron microscope
C) brightfield microscope
D) transmission electron microscope
E) darkfield microscope

F) A) and B)
G) B) and E)

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Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

A) True
B) False

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Which of the following pairs is mismatched?


A) confocal microscope -produces a three- dimensional image
B) fluorescence microscope -uses a fluorescent light
C) scanning electron microscope -produces a three- dimensional image
D) darkfield microscope -uses visible light
E) scanning tunneling microscope -allows visualization of atoms

F) A) and C)
G) B) and E)

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What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?


A) 45x
B) 100x
C) 4.5x
D) 10x
E) 450x

F) A) and D)
G) C) and D)

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A microorganism measures 5 ยตm in length. Its length in mm would be


A) 50 mm.
B) 0.005 mm.
C) 0.5 mm.
D) 500 mm.
E) 0.05 mm.

F) B) and C)
G) A) and C)

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The capsules and flagella of bacteria can be observed in gram- stained smears.

A) True
B) False

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