Question 1

Bioremediation has been used to treat which of the following?

Accepted Answer

A)  Oil spills
B)  Sewage
C)  Pesticides
D)  Heavy metals
E)  All of the answers have been successfully remediatedF) B) and D)
G) B) and E)

Question 2

Which of the following is an example of a pluripotent cell?

Accepted Answer

A)   embryonic stem cells
B)   red blood cells
C)   fetal heart cells
D)   umbilical cord blood
E)   nerve cellsF) A) and D)
G) B) and E)

Question 3

Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text) , which of the following would identify those mouse cells that were the result of homologous recombination?

Accepted Answer

A)  NeoR only
B)  Tk only
C)  NeoR and Tk
D)  Neither NeoR or TkE) A) and D)
F) All of the above

Question 4

Unipotent cells may differentiate into all other cell types of the body.

Accepted Answer

A) True 
 B)False

Question 5

Which of the following terms represents a cell that can form any other cell of the organism?

Accepted Answer

A)  Pluripotent
B)  Totipotent
C)  UnipotentD) A) and B)
E) B) and C) B

Question 6

The first organism ever cloned by researchers was Dolly the sheep in 1997.

Accepted Answer

A) True 
 B)False

Question 7

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Accepted Answer

A)  So that it was compatible with the restriction enzyme
B)  As a selectable marker
C)  To allow the somatostatin to link to a bacterial protein
D)  To increase the length of the peptide to make it more commercialE) C) and D)
F) B) and D)

Question 8

Which of the following is not an advantage of a transgenic plant?

Accepted Answer

A)  Widely accepted by the public
B)  Increased resistance to disease and pests
C)  Adaptability to harsh environments
D)  Improved shelf lifeE) A) and D)
F) A) and C)

Question 9

A transgenic organism has recombinant DNA integrated into its genome.

Accepted Answer

A) True 
 B)False  True

Question 10

Identical twins are an example of gene cloning.

Accepted Answer

A) True 
 B)False

Question 11

What is the purpose of the cyanogen bromide (CNBr)  in the manufacture of insulin and somatostatin by recombinant bacteria?

Accepted Answer

A)  Allows for the uptake of the plasmid
B)  Enhances the activity of the restriction enzymes
C)  Creates sticky ends for integration into the plasmid
D)  Cleaves the protein from the b-galactosidase proteinE) All of the above
F) B) and C)

Question 12

An individual who contains cells that are mutated is called a chimera.

Accepted Answer

A) True 
 B)False  False

Question 13

A major disadvantage with current gene therapy techniques using viral vectors is the possibility of an immune response against the virus.

Accepted Answer

A) True 
 B)False

Question 14

In the production of transgenic plants, what selectable marker is most commonly used to indicate that a cell has taken up the T DNA that includes the cloned gene?

Accepted Answer

A)  Ampicillin
B)  Neomycin
C)  Streptomycin
D)  KanamycinE) B) and C)
F) All of the above

Question 15

An organism that can be regenerated by somatic cells is called multipotent.

Accepted Answer

A) True 
 B)False

Question 16

Although Dolly was only three years old, her chromosomes had the length of a 9-10 year old sheep. Which of the following best describes why this occurred?

Accepted Answer

A)  The cell lines that created Dolly were aged prematurely in the lab
B)  Nonhomologous recombination
C)  A mutation enhanced the rate of aging
D)  Dolly was not a clone
E)  The telomeres of the somatic cells that Dolly originated from were shorteningF) B) and C)
G) A) and E)

Question 17

Hematopoietic stem cells are _________.

Accepted Answer

A)  Pluripotent
B)  Totipotent
C)  Unipotent
D)  MultipotentE) A) and B)
F) None of the above

Question 18

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following mechanisms uses high-velocity microprojectiles, coated with DNA, to produce a transgenic plant?

Accepted Answer

A)  A.tumefaciens
B)  Electroporation
C)  Microinjection
D)  Biolistic gene transferE) C) and D)
F) B) and D)

Question 20

The first human diseases that was successfully treated using gene therapy was ______.

Accepted Answer

A)   Diabetes
B)   Cystic fibrosis
C)   Severe combined immunodeficiency diseases
D)   Muscular dystrophyE) A) and D)
F) B) and D)

Exam 21: Biotechnology

Bioremediation has been used to treat which of the following?

Correct AnswerverifiedShow Answer

Which of the following is an example of a pluripotent cell?

Correct AnswerverifiedShow Answer

Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text) , which of the following would identify those mouse cells that were the result of homologous recombination?

Correct AnswerverifiedShow Answer

Unipotent cells may differentiate into all other cell types of the body.

Correct AnswerverifiedShow Answer

Which of the following terms represents a cell that can form any other cell of the organism?

Correct AnswerverifiedB

The first organism ever cloned by researchers was Dolly the sheep in 1997.

Correct AnswerverifiedShow Answer

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Correct AnswerverifiedShow Answer

Which of the following is not an advantage of a transgenic plant?

Correct AnswerverifiedShow Answer

A transgenic organism has recombinant DNA integrated into its genome.

Correct AnswerverifiedTrue

Identical twins are an example of gene cloning.

Correct AnswerverifiedShow Answer

What is the purpose of the cyanogen bromide (CNBr) in the manufacture of insulin and somatostatin by recombinant bacteria?

Correct AnswerverifiedShow Answer

An individual who contains cells that are mutated is called a chimera.

Correct AnswerverifiedFalse

A major disadvantage with current gene therapy techniques using viral vectors is the possibility of an immune response against the virus.

Correct AnswerverifiedShow Answer

In the production of transgenic plants, what selectable marker is most commonly used to indicate that a cell has taken up the T DNA that includes the cloned gene?

Correct AnswerverifiedShow Answer

An organism that can be regenerated by somatic cells is called multipotent.

Correct AnswerverifiedShow Answer

Although Dolly was only three years old, her chromosomes had the length of a 9-10 year old sheep. Which of the following best describes why this occurred?

Correct AnswerverifiedShow Answer

Hematopoietic stem cells are _________.

Correct AnswerverifiedShow Answer

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

Correct AnswerverifiedShow Answer

Which of the following mechanisms uses high-velocity microprojectiles, coated with DNA, to produce a transgenic plant?

Correct AnswerverifiedShow Answer

The first human diseases that was successfully treated using gene therapy was ______.

Correct AnswerverifiedShow Answer

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Using the Neo^R/Tk system of introducing gene replacements into mice (as described in the text) , which of the following would identify those mouse cells that were the result of homologous recombination?

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B

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True

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False

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