Question 1

You are working in a cancer lab and are interested in identifying some genes that are important in the onset of lung cancer.You have several genes you think may be involved,so you "print" spots of the candidate DNA sequences on a microarray slide.After doing this,you isolate mRNA from both normal lung cells and cancerous lung cells.You make fluorescently-tagged cDNA from each mRNA sample;the cDNAs from normal cells are tagged with a green-colored molecule and the cDNAs from cancer cells are tagged with a red-colored molecule.You hybridize the cDNAs with the microarray and get the following data.(Green spots are labeled with "G",red spots are labeled with "R") . 
What spots represent DNA that you should study further as potentially having a role in lung cancer?

Accepted Answer

A)  Spots A1 and C6
B)  Spots B4 and B6
C)  Spots A1,B4,B6,and C6
D)  All spots except A1 and C6
E)  All spots except A1,B4,B6,and C6F) D) and E)
G) B) and D) A

Question 2

After a fragment of DNA containing the gene of interest has been inserted into a vector,how are the gaps between the two pieces of DNA sealed together?

Accepted Answer

A)  DNA polymerase catalyzes the formation of hydrogen bonds in the DNA backbone.
B)  DNA gyrase creates tension in the DNA backbone to hold the molecule together.
C)  DNA helicase causes the double helix to wind into its stable form.
D)  DNA ligase catalyzes the formation of covalent bonds in the DNA backbone.
E)  Restriction enzymes have the ability to join the two DNA fragments.F) All of the above
G) A) and B)

Question 3

What field of science is a researcher practicing if she is conducting experiments which attempt to map the location of a gene on a particular chromosome?

Accepted Answer

A)  genomics
B)  reproductive genomics
C)  functional genomics
D)  proteomics
E)  electrophoresisF) B) and D)
G) B) and E)

Question 4

A researcher wants to introduce the human gene encoding tissue plasminogen activator (used to dissolve blood clots) into a mammal so that the protein will be secreted into the milk of the mammary gland.What is required for the researcher's success?

Accepted Answer

A)  The gene should be placed a considerable distance away from the promoter of a gene that is expressed in mammary cells
B)  The gene should be placed next to the promoter of a gene that is expressed in mammary cells
C)  The gene should be inserted in place of the promoter of a gene that is expressed in mammary cells
D)  The gene should be placed next to the promoter of a gene that is expressed in multiple types of cells
E)  The gene should be inserted in place of the promoter of a gene that is expressed in multiple types of cellsF) None of the above
G) A) and B)

Question 5

In order to produce human insulin in bacteria what must be done first?

Accepted Answer

A)  The human insulin gene must be transformed into bacteriA.
B)  Bacteria containing the human insulin gene are grown.
C)  The coding sequence of the genes which produce human insulin must be isolated.
D)  The bacterial insulin gene must be cloneD.
E)  Human insulin protein is purified from the bacteria.F) C) and E)
G) None of the above

Question 6

The milk of goats has been genetically modified to produce human antithrombin,a protein with anticoagulant properties.How did the recombinant plasmid containing the human antithrombin gene get into the goats?

Accepted Answer

A)  the goats ingested bacterial pellets containing the recombinant plasmid,the DNA then integrated into their cells
B)  the recombinant plasmid was injected into mammary cells
C)  the recombinant plasmid was put into a goat oocyte,which was in turn fertilized and implanted into a surrogate goat mother
D)  the recombinant plasmid was put into a viral vector;the virus then infected the goat cells
E)  the goat cells were treated with chemical agents such as calcium chloride,which made them competent to take up DNAF) A) and D)
G) A) and E)

Question 7

Dideoxy nucleotides are used in which of the following processes?

Accepted Answer

A)  Microarray analysis
B)  DNA sequencing
C)  PCR
D)  Transformation
E)  making a recombinant plasmidF) C) and E)
G) B) and E)

Question 8

The lacZ gene encodes

Accepted Answer

A)  aromatase
B)  glucose dehydrogenase
C)  ampicillin-resistance protein
D)  b-galactosidase
E)  lactoseF) B) and D)
G) C) and D)

Question 9

In a DNA microarray,_____ are attached to the slide and ___ are fluorescently labeled.

Accepted Answer

A)  the cDNAs,the genes
B)  the mRNAs,the genes
C)  the samples,the cDNAs
D)  the samples,the mRNAs
E)  the nucleotides,the cellsF) A) and E)
G) C) and E)

Question 10

Which of the following sequence pairs is a palindrome?

Accepted Answer

A)  5'-TCACGT-3'3'-TCACGT-5'
B)  5'-GAATCG-3'3'-CTTAGC-5'
C)  None of the paired sequences are a palindrome.
D)  5'-GTTCAT-3'3'-CAAGTA-5'
E)  5'-TCCGGA-3'F) A) and B)
G) C) and E)

Question 11

You transform bacteria with a plasmid carrying the ampicillin-resistance gene ampR. How would you determine which bacteria took up the plasmid?

Accepted Answer

A)  Bacteria containing the plasmid would be able to grow in the absence of ampicillin.
B)  Ampicillin-resistance is necessary for the plasmid DNA to get into the bacteria.
C)  Bacteria containing the plasmid would be killed in the presence of ampicillin.
D)  Bacteria containing the plasmid would be able to produce ampicillin.
E)  Bacteria containing the plasmid would be able to grow in the presence of ampicillin.F) B) and D)
G) A) and E)

Question 12

A DNA library is

Accepted Answer

A)  a collection of recombinant vectors containing DNA fragments of a given organism
B)  a collection of books about clones
C)  a line of bacteria that contains a DNA fragment of interest
D)  a series of restriction enzymes that can be used to produce a variety of sizes of DNA fragments
E)  a collection of recombinant plasmids containing a particular gene from many different organismsF) A) and B)
G) A) and C)

Question 13

What is an advantage of cDNA libraries over genomic libraries?

Accepted Answer

A)  cDNA libraries are more stable than genomic libraries.
B)  cDNA lacks exons.
C)  cDNA libraries require fewer steps to make than genomic libraries.
D)  cDNA only contains protein coding exons.
E)  cDNA libraries are cheaper to make than genomic libraries.F) D) and E)
G) A) and B) D

Question 14

Chromosomes within somatic cells of mammals cannot be cloned because they accumulate irreversible genetic changes.

Accepted Answer

A) True 
 B)False

Question 15

You cut some sheep DNA and some plasmid DNA with the same restriction enzyme.The plasmid contains an AmpR gene and a lacZ gene.You mix together the fragments of sheep DNA and the linearized plasmid.You transform bacteria with your mixture,and plate the bacterial cells on growth media containing the antiobiotic ampicillin and X-gal (the substrate of beta-galactosidase) .You need to pick the bacterial colonies that contain recombinant plasmids.Which colonies do you choose?

Accepted Answer

A)  only the blue colonies - these contain recombinant plasmids
B)  only the white colonies - these contain recombinant plasmids
C)  the largest colonies - these are the most resistant to ampicillin and thus more likely to contain a recombinant plasmids
D)  all colonies will contain plasmids,but additional testing is needed to determine if the plasmids are recombinant
E)  all surviving colonies;if they grow on the media they are ampicillin-resistant and contain recombinant plasmidsF) A) and E)
G) None of the above B

Question 16

How would you accurately summarize the process used to make Dolly the sheep?

Accepted Answer

A)  Mitochondrial DNA was taken from one female and injected into the mammary glands of another female.
B)  A sheep embryo was produced by in vitro fertilization.At an early stage,the cells of the embryo were separated,and each cells was put into a surrogate sheep mother.
C)  A mammary cell of one sheep was fused with an enucleated egg of another sheep.The resulting diploid embryo was placed in a surrogate sheep mother.
D)  A set of twins - one male and one female - were mateD.Since the twins had identical DNA,their lamb was a clone.
E)  The sheep to be cloned was given a drug that induced the cells of her ovary to divide by binary fission.The resulting diploid eggs did not need to be fertilized,and were used to produce sheep embryos,including Dolly.F) D) and E)
G) A) and C)

Question 17

The first human gene therapy was approved for patients suffering ADA (adenosine deaminase) deficiency.In this therapy,lymphocytes were removed from the patient,the normal ADA gene was put into the cells,and the cells were returned to the bloodstream.How was the normal ADA put into the lymphocytes?

Accepted Answer

A)  via the polymerase chain reaction
B)  via a plasmid vector
C)  microinjection
D)  by fusing normal lymphocytes with deficient lymphocytes
E)  via a viral vectorF) A) and D)
G) B) and E)

Question 18

DNA microarrays (gene chips)are only capable of monitoring the expression of one gene at a time.

Accepted Answer

A) True 
 B)False

Question 19

If you have a double stranded synthetic piece of DNA that you want to attach to a PCR product,what enzyme would you use?

Accepted Answer

A)  Taq DNA polymerase
B)  reverse transcriptase
C)  DNA ligase
D)  DNA polymerase
E)  restriction enzymeF) A) and C)
G) A) and B)

Question 20

The main purpose of polymerase chain reaction (PCR)is to generate many copies of a region of DNA.

Accepted Answer

A) True 
 B)False

Exam 16: Genetic Technology

Correct Answer
verified
A

After a fragment of DNA containing the gene of interest has been inserted into a vector,how are the gaps between the two pieces of DNA sealed together?

Correct Answer
verified

What field of science is a researcher practicing if she is conducting experiments which attempt to map the location of a gene on a particular chromosome?

Correct Answer
verified

A researcher wants to introduce the human gene encoding tissue plasminogen activator (used to dissolve blood clots) into a mammal so that the protein will be secreted into the milk of the mammary gland.What is required for the researcher's success?

Correct Answer
verified

In order to produce human insulin in bacteria what must be done first?

Correct Answer
verified

The milk of goats has been genetically modified to produce human antithrombin,a protein with anticoagulant properties.How did the recombinant plasmid containing the human antithrombin gene get into the goats?

Correct Answer
verified

Dideoxy nucleotides are used in which of the following processes?

Correct Answer
verified

The lacZ gene encodes

Correct Answer
verified

In a DNA microarray,___ are attached to the slide and _ are fluorescently labeled.

Correct Answer
verified

Which of the following sequence pairs is a palindrome?

Correct Answer
verified

You transform bacteria with a plasmid carrying the ampicillin-resistance gene amp^R.How would you determine which bacteria took up the plasmid?

Correct Answer
verified

A DNA library is

Correct Answer
verified

What is an advantage of cDNA libraries over genomic libraries?

Correct Answer
verified
D

Chromosomes within somatic cells of mammals cannot be cloned because they accumulate irreversible genetic changes.

Correct Answer
verified

Correct Answer
verified
B

How would you accurately summarize the process used to make Dolly the sheep?

Correct Answer
verified

The first human gene therapy was approved for patients suffering ADA (adenosine deaminase) deficiency.In this therapy,lymphocytes were removed from the patient,the normal ADA gene was put into the cells,and the cells were returned to the bloodstream.How was the normal ADA put into the lymphocytes?

Correct Answer
verified

DNA microarrays (gene chips)are only capable of monitoring the expression of one gene at a time.

Correct Answer
verified

If you have a double stranded synthetic piece of DNA that you want to attach to a PCR product,what enzyme would you use?

Correct Answer
verified

The main purpose of polymerase chain reaction (PCR)is to generate many copies of a region of DNA.

Correct Answer
verified

Exam 16: Genetic Technology

Correct AnswerverifiedA

After a fragment of DNA containing the gene of interest has been inserted into a vector,how are the gaps between the two pieces of DNA sealed together?

Correct AnswerverifiedShow Answer

What field of science is a researcher practicing if she is conducting experiments which attempt to map the location of a gene on a particular chromosome?

Correct AnswerverifiedShow Answer

A researcher wants to introduce the human gene encoding tissue plasminogen activator (used to dissolve blood clots) into a mammal so that the protein will be secreted into the milk of the mammary gland.What is required for the researcher's success?

Correct AnswerverifiedShow Answer

In order to produce human insulin in bacteria what must be done first?

Correct AnswerverifiedShow Answer

The milk of goats has been genetically modified to produce human antithrombin,a protein with anticoagulant properties.How did the recombinant plasmid containing the human antithrombin gene get into the goats?

Correct AnswerverifiedShow Answer

Dideoxy nucleotides are used in which of the following processes?

Correct AnswerverifiedShow Answer

The lacZ gene encodes

Correct AnswerverifiedShow Answer

In a DNA microarray,_____ are attached to the slide and ___ are fluorescently labeled.

Correct AnswerverifiedShow Answer

Which of the following sequence pairs is a palindrome?

Correct AnswerverifiedShow Answer

You transform bacteria with a plasmid carrying the ampicillin-resistance gene ampR. How would you determine which bacteria took up the plasmid?

Correct AnswerverifiedShow Answer

A DNA library is

Correct AnswerverifiedShow Answer

What is an advantage of cDNA libraries over genomic libraries?

Correct AnswerverifiedD

Chromosomes within somatic cells of mammals cannot be cloned because they accumulate irreversible genetic changes.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedB

How would you accurately summarize the process used to make Dolly the sheep?

Correct AnswerverifiedShow Answer

The first human gene therapy was approved for patients suffering ADA (adenosine deaminase) deficiency.In this therapy,lymphocytes were removed from the patient,the normal ADA gene was put into the cells,and the cells were returned to the bloodstream.How was the normal ADA put into the lymphocytes?

Correct AnswerverifiedShow Answer

DNA microarrays (gene chips)are only capable of monitoring the expression of one gene at a time.

Correct AnswerverifiedShow Answer

If you have a double stranded synthetic piece of DNA that you want to attach to a PCR product,what enzyme would you use?

Correct AnswerverifiedShow Answer

The main purpose of polymerase chain reaction (PCR)is to generate many copies of a region of DNA.

Correct AnswerverifiedShow Answer

Correct Answer
verified
A

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

In a DNA microarray,___ are attached to the slide and _ are fluorescently labeled.

Correct Answer
verified

Correct Answer
verified

You transform bacteria with a plasmid carrying the ampicillin-resistance gene amp^R.How would you determine which bacteria took up the plasmid?

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
D

Correct Answer
verified

Correct Answer
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B

Correct Answer
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Correct Answer
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