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Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis

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Question 1

Multiple Choice

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Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

A) streak plate
B) spread plate
C) pour plate
D) All of the choices are correct.
E) None of the choices is correct.

F) B) and C)
G) B) and D)

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Question 2

Multiple Choice

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A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

A) negative staining.
B) using an acidic dye.
C) simple staining.
D) using the acid-fast stain.
E) capsule staining.

F) B) and C)
G) C) and E)

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Question 3

Multiple Choice

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Agar is a complex polysaccharide that comes from a(n)

The specimen preparation that is best for viewing cell motility is

Which of the following is not a benefit of agar as a solid medium?

Mixed cultures are also referred to as contaminated cultures.

All of the following pertain to the fluorescence microscope except it

Newly inoculated cultures must be _____ at a specific temperature to encourage growth.

A pure culture contains

Which of the following magnifies the specimen to produce its virtual image?

Please read the clinical scenario, and then answer the questions that follow to become familiar with the traditional NCLEX question format. Ms. Hungh, a Burmese immigrant, enters your clinic with her interpreter complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking assistance for her condition. -A sputum sample is ordered for microbial analysis in order to rule out the diagnosis of tuberculosis.Suspecting Mycobacterium tuberculosis may be the pathogen,you know that the laboratory technicians will perform which stain on the sample?

What type of isolation technique is most effective for the majority of applications?

All of the following are diameters of cells that would be resolved in a microscope with a 0.2 µm limit of resolution except

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing. -Gram stain results from the patient specimen indicate a bacterial infection.From your microbiology course,you remember that this staining procedure involves

If a microbiologist is studying a specimen at a total magnification of 950x,what is the magnifying power of the objective lens if the ocular lens is 10x?

Differential media results in which of the following growth characteristics?

The type of microscope in which you would see brightly illuminated specimens against a black background is

The real image is the reverse of the actual specimen.

Please read the clinical scenario, and then answer the questions that follow to become familiar with the traditional NCLEX question format. Ms. Hungh, a Burmese immigrant, enters your clinic with her interpreter complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking assistance for her condition. -Culturing of the sputum resulted in the growth of distinct colonies on the medium,and the technician informs you that further isolation by subculturing is now needed.You understand that this is accomplished by taking a bit of growth from an isolated colony and inoculating a separate medium,resulting in the production of a

A nutrient medium that contains at least one ingredient that is NOT chemically definable would be termed