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Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?


A) Ouchterlony double diffusion
B) Western blot
C) immunelectrophoresis
D) radioimmunoassay (RIA)
E) the Quellung test

F) B) and D)
G) A) and B)

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You place an inoculum of your bacterium on a glass slide and add a drop of hydrogen peroxide reagent.Promptly you see bubbles appear,indicating the presence of a particular enzyme that the bacterium produces.What type of identification test does this exemplify?


A) biochemical test
B) microarray
C) precipitation test
D) immunochromatography

E) B) and C)
F) All of the above

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Titer is the amount of _____ in serum.


A) antigen
B) antibody
C) WBC
D) complement
E) memory cells

F) D) and E)
G) C) and E)

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Soluble antigens are detected in this type of test:


A) cross-reactions
B) agglutination
C) precipitation
D) specificity
E) sensitivity

F) All of the above
G) A) and E)

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If you were going to identify microorganisms within a specimen containing large numbers of organisms (seawater,body flora,etc. ) ,you would have to:


A) use different kinds of serological tests.
B) use a wide variety of microbiological media to identify all of the different species of microorganisms.
C) use a wide variety of antibodies to react with the antigens.
D) amplify gene sequences within the sample to identify each of the different organisms.

E) None of the above
F) All of the above

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Which of the following is not a phenotypic method of identification?


A) morphology
B) Gram stain reaction
C) acid-fast reaction
D) antibody response
E) endospore production

F) A) and E)
G) A) and B)

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Which of the following is correct regarding the polymerse chain reaction test?


A) First antigen and antibody are allowed to react.
B) An enzyme-linked indicator is added.
C) Sheep red blood cells are added to the gel.
D) The two strands of the DNA molecules are separated by heat.

E) A) and B)
F) All of the above

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The dyes fluorescein and rhodamine


A) can be used to label antibodies in immunofluorescence tests.
B) emit visible light in response to ultraviolet radiation.
C) are observed using fluorescence microscopy.
D) are used to identify pathogens of chlamydiosis,Legionnaires' disease,and others.
E) All of the choices are correct.

F) A) and D)
G) C) and D)

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A serum titer involves


A) quantifying the number of infectious particles in a specimen.
B) determining the lowest dilution of serum that produces a visible reaction.
C) determining the highest dilution of serum that produces a visible reaction.
D) the Western blot method.

E) A) and B)
F) B) and C)

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The property of a test to detect only a certain antibody or antigen and not to react with any others is


A) cross-reaction.
B) agglutination.
C) precipitation.
D) specificity.
E) sensitivity.

F) A) and D)
G) None of the above

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All of the following are routinely collected for microbial analysis of a specimen except:


A) saliva.
B) skin.
C) spinal fluid.
D) hair.
E) blood.

F) C) and D)
G) None of the above

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Specimen collection


A) is always done by a medical professional.
B) must be done under sterile conditions.
C) must utilize aseptic techniques.
D) does not require special handling.

E) A) and B)
F) C) and D)

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Direct antigen testing differs from direct fluorescence testing in that the results


A) are read with the naked eye.
B) require specialized equipment.
C) are more accurate.
D) are less accurate.
E) require an electron microscope.

F) B) and E)
G) None of the above

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What type of test will detect cell-associated antigens?


A) cross-reaction
B) agglutination
C) precipitation
D) specificity
E) sensitivity

F) A) and E)
G) B) and C)

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